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A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen sectio...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Tajika, Yuki, Murakami, Tohru, Iijima, Keiya, Gotoh, Hiroki, Takahashi-Ikezawa, Maiko, Ueno, Hitoshi, Yoshimoto, Yuhei, Yorifuji, Hiroshi
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5473845/
https://ncbi.nlm.nih.gov/pubmed/28623366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03900-9
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