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Propagation of Rhinovirus on WI-38 Cell Monolayers in Rolling Bottles

Techniques are described for the propagation of rhinoviruses on WI-38 monolayers in rolling bottles. High yields of viruses were obtained, as indicated by infectivity titers and electron microscopy. When crude harvests were subjected to low-speed centrifugation and then filtered through a 0.45-μ mem...

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Bibliographic Details
Main Authors: Fenters, James D., Fordyce, Patricia A., Gerin, John L., Holper, Jacob C.
Format: Artigo
Language:Inglês
Published: 1967
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC547234/
https://ncbi.nlm.nih.gov/pubmed/16349766
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