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Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers

The structure of the p7 viroporin, an oligomeric membrane protein ion channel involved in the assembly and release of the hepatitis C virus, was determined from proteins expressed and inserted directly into supported model lipid membranes using cell-free protein expression. Cell-free protein express...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Soranzo, Thomas, Martin, Donald K., Lenormand, Jean-Luc, Watkins, Erik B.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5469739/
https://ncbi.nlm.nih.gov/pubmed/28611396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03472-8
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