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New Strategy for Identification of Novel cry-Type Genes from Bacillus thuringiensis Strains
We designed five degenerate primers for detection of novel cry genes from Bacillus thuringiensis strains. An efficient strategy was developed based on a two-step PCR approach with these primers in five pair combinations. In the first step, only one of the primer pairs is used in the PCR, which allow...
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology
2005
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC546719/ https://ncbi.nlm.nih.gov/pubmed/15691928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.761-765.2005 |
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