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New Strategy for Identification of Novel cry-Type Genes from Bacillus thuringiensis Strains

We designed five degenerate primers for detection of novel cry genes from Bacillus thuringiensis strains. An efficient strategy was developed based on a two-step PCR approach with these primers in five pair combinations. In the first step, only one of the primer pairs is used in the PCR, which allow...

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Bibliografiset tiedot
Päätekijät: Berón, Corina M., Curatti, Leonardo, Salerno, Graciela L.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology 2005
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC546719/
https://ncbi.nlm.nih.gov/pubmed/15691928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.761-765.2005
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