Comparison of Ileum Microflora of Pigs Fed Corn-, Wheat-, or Barley-Based Diets by Chaperonin-60 Sequencing and Quantitative PCR

We have combined the culture-independent methods of high-throughput sequencing of chaperonin-60 PCR product libraries and quantitative PCR to profile and quantify the small-intestinal microflora of pigs fed diets based on corn, wheat, or barley. A total of 2,751 chaperonin-60 PCR product clones prod...

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Autors principals: Hill, Janet E., Hemmingsen, Sean M., Goldade, Blair G., Dumonceaux, Tim J., Klassen, Jonathan, Zijlstra, Ruurd T., Goh, Swee Han, Van Kessel, Andrew G.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Microbial Ecology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC546709/
https://ncbi.nlm.nih.gov/pubmed/15691942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.867-875.2005
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