Chondroitin sulphate extracted from antler cartilage using high hydrostatic pressure and enzymatic hydrolysis
Chondroitin sulphate (CS), a major glycosaminoglycan, is an essential component of the extracellular matrix in cartilaginous tissues. Wapiti velvet antlers are a rich source of these molecules. The purpose of the present study was to develop an effective isolation procedure of CS from fresh velvet a...
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| Xuất bản năm: | Biotechnol Rep (Amst) |
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| Những tác giả chính: | , , , , |
| Định dạng: | Artigo |
| Ngôn ngữ: | Inglês |
| Được phát hành: |
Elsevier
2014
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| Những chủ đề: | |
| Truy cập trực tuyến: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5466122/ https://ncbi.nlm.nih.gov/pubmed/28626657 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2014.07.004 |
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| Résumé: | Chondroitin sulphate (CS), a major glycosaminoglycan, is an essential component of the extracellular matrix in cartilaginous tissues. Wapiti velvet antlers are a rich source of these molecules. The purpose of the present study was to develop an effective isolation procedure of CS from fresh velvet antlers using a combination of high hydrostatic pressure (100 MPa) and enzymatic hydrolysis (papain). High CS extractability (95.1 ± 2.5%) of total uronic acid was obtained following incubation (4 h at 50 °C) with papain at pH 6.0 in 100 MPa compared to low extractability (19 ± 1.1%) in ambient pressure (0.1 MPa). Antler CS fractions were isolated by Sephacryl S-300 chromatography and identified by western blot using an anti-CS monoclonal antibody. The antler CS fraction did not aggregate with hyaluronic acid in CL-2B chromatography and possessed DPPH radical scavenging activity at 78.3 ± 1.5%. The results indicated that high hydrostatic pressure and enzymatic hydrolysis procedure may be a useful tool for the isolation of CS from antler cartilaginous tissues. |
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