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Detection of genome-edited mutant clones by a simple competition-based PCR method
Genome editing by the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) system is a revolutionary strategy to study gene functions. Since the efficiency of gene disruption in cell culture does not reach 100% typically, cloning of mutant cells is of...
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| Published in: | PLoS One |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Public Library of Science
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5460891/ https://ncbi.nlm.nih.gov/pubmed/28586390 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179165 |
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