Loading...

Detection of genome-edited mutant clones by a simple competition-based PCR method

Genome editing by the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) system is a revolutionary strategy to study gene functions. Since the efficiency of gene disruption in cell culture does not reach 100% typically, cloning of mutant cells is of...

Full description

Saved in:
Bibliographic Details
Published in:PLoS One
Main Authors: Harayama, Takeshi, Riezman, Howard
Format: Artigo
Language:Inglês
Published: Public Library of Science 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5460891/
https://ncbi.nlm.nih.gov/pubmed/28586390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179165
Tags: Add Tag
No Tags, Be the first to tag this record!