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General colorimetric method for DNA diagnostics allowing direct solid-phase genomic sequencing of the positive samples.

A system for rapid colorimetric detection of specific genome DNA fragments amplified by the polymerase chain reaction (PCR) is described that has been designed to allow direct solid-phase sequencing of positive samples. The amplified material is immobilized on magnetic beads by using the biotin stre...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Wahlberg, J, Lundeberg, J, Hultman, T, Uhlén, M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1990
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54578/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2118652/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.17.6569
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