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Combined Inactivation and Expression Strategy To Study Gene Function under Physiological Conditions: Application to Identification of New Escherichia coli Adhesins
In bacteria, whereas disruption methods have been improved recently, the use of plasmid complementation strategies are still subject to limitations, such as cloning difficulties, nonphysiological levels of gene expression, or a requirement for antibiotics as plasmid selection pressure. Moreover, bec...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC545717/ https://ncbi.nlm.nih.gov/pubmed/15659678 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.3.1001-1013.2005 |
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