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Combined Inactivation and Expression Strategy To Study Gene Function under Physiological Conditions: Application to Identification of New Escherichia coli Adhesins

In bacteria, whereas disruption methods have been improved recently, the use of plasmid complementation strategies are still subject to limitations, such as cloning difficulties, nonphysiological levels of gene expression, or a requirement for antibiotics as plasmid selection pressure. Moreover, bec...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Roux, Agnès, Beloin, Christophe, Ghigo, Jean-Marc
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC545717/
https://ncbi.nlm.nih.gov/pubmed/15659678
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.3.1001-1013.2005
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