Protein Isoaspartate Methyltransferase Is a Multicopy Suppressor of Protein Aggregation in Escherichia coli

We used preS2-S′-β-galactosidase, a three-domain fusion protein that aggregates extensively at 43°C in the cytoplasm of Escherichia coli, to search for multicopy suppressors of protein aggregation and inclusion body formation and took advantage of the known differential solubility of preS2-S′-β-gala...

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Bibliographic Details
Main Authors: Kern, Renée, Malki, Abderrahim, Abdallah, Jad, Liebart, Jean-Claude, Dubucs, Catherine, Yu, Myeong Hee, Richarme, Gilbert
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
Subjects:
Enzymes and Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC545615/
https://ncbi.nlm.nih.gov/pubmed/15687202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.4.1377-1383.2005
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