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Substrate specificity and kinetic framework of a DNAzyme with an expanded chemical repertoire: a putative RNaseA mimic that catalyzes RNA hydrolysis independent of a divalent metal cation

This work addresses the binding, cleavage and dissociation rates for the substrate and products of a synthetic RNaseA mimic that was combinatorially selected using chemically modified nucleoside triphosphates. This trans-cleaving DNAzyme, 9(25)-11t, catalyzes sequence-specific ribophosphodiester hyd...

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Detalhes bibliográficos
Main Authors: Ting, Richard, Thomas, Jason M., Lermer, Leonard, Perrin, David M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC545449/
https://ncbi.nlm.nih.gov/pubmed/15625232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh1007
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