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An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states

Macromolecular crowding ought to stabilize folded forms of proteins, through an excluded volume effect. This explanation has been questioned and observed effects attributed to weak interactions with other cell components. Here we show conclusively that protein stability is affected by volume exclusi...

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Bibliographische Detailangaben
Veröffentlicht in:Nat Commun
Hauptverfasser: Alfano, Caterina, Sanfelice, Domenico, Martin, Stephen R., Pastore, Annalisa, Temussi, Piero Andrea
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5454340/
https://ncbi.nlm.nih.gov/pubmed/28516908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15428
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