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An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states

Macromolecular crowding ought to stabilize folded forms of proteins, through an excluded volume effect. This explanation has been questioned and observed effects attributed to weak interactions with other cell components. Here we show conclusively that protein stability is affected by volume exclusi...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Alfano, Caterina, Sanfelice, Domenico, Martin, Stephen R., Pastore, Annalisa, Temussi, Piero Andrea
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5454340/
https://ncbi.nlm.nih.gov/pubmed/28516908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms15428
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