Optimized multiplex immunofluorescence single-cell analysis reveals tuft cell heterogeneity

Intestinal tuft cells are a rare, poorly understood cell type recently shown to be a critical mediator of type 2 immune response to helminth infection. Here, we present advances in segmentation algorithms and analytical tools for multiplex immunofluorescence (MxIF), a platform that enables iterative...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:JCI Insight
Main Authors: McKinley, Eliot T., Sui, Yunxia, Al-Kofahi, Yousef, Millis, Bryan A., Tyska, Matthew J., Roland, Joseph T., Santamaria-Pang, Alberto, Ohland, Christina L., Jobin, Christian, Franklin, Jeffrey L., Lau, Ken S., Gerdes, Michael J., Coffey, Robert J.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Clinical Investigation 2017
Teme:
Technical Advance
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5453701/
https://ncbi.nlm.nih.gov/pubmed/28570279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1172/jci.insight.93487
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