Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses

We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro...

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Publicat a:Mem Inst Oswaldo Cruz
Autors principals: Naveca, Felipe Gomes, do Nascimento, Valdinete Alves, de Souza, Victor Costa, Nunes, Bruno Tardelli Diniz, Rodrigues, Daniela Sueli Guerreiro, Vasconcelos, Pedro Fernando da Costa
Format: Artigo
Idioma:Inglês
Publicat: Instituto Oswaldo Cruz, Ministério da Saúde 2017
Matèries:
Technical Note
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5452489/
https://ncbi.nlm.nih.gov/pubmed/28591313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/0074-02760160062
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