Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

BACKGROUND: Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. However, t...

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Detalhes bibliográficos
Publicado no:Malar J
Main Authors: Echeverry, Diego F., Deason, Nicholas A., Makuru, Victoria, Davidson, Jenna, Xiao, Honglin, Niedbalski, Julie, Yu, Xiaoyu, Stevenson, Jennifer C., Bugoro, Hugo, Aparaimo, Allan, Reuben, Hedrick, Cooper, Robert, Burkot, Thomas R., Russell, Tanya L., Collins, Frank H., Lobo, Neil F.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5452387/
https://ncbi.nlm.nih.gov/pubmed/28569159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12936-017-1881-1
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