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Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging

Fluorescence live-cell imaging by single molecule localization microscopy (SMLM) or fluorescence lifetime imaging microscopy (FLIM) in principle allows for the spatio-temporal observation of molecular patterns in individual, living cells. However, the dynamics of molecules within cells hamper their...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Huebinger, Jan, Masip, Martin E., Christmann, Jens, Bastiaens, Philippe I. H.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5450929/
https://ncbi.nlm.nih.gov/pubmed/28580376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2236
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