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Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection
Delivery of DNA to cells and its subsequent integration into the host genome is a fundamental task in molecular biology, biotechnology and gene therapy. Here we describe an IP-free one-step method that enables stable genome integration into either prokaryotic or eukaryotic cells. A synthetic mariner...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5449632/ https://ncbi.nlm.nih.gov/pubmed/28204586 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx113 |
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