Examination of Enterococcus faecalis Toxin-Antitoxin System Toxin Fst Function Utilizing a Pheromone-Inducible Expression Vector with Tight Repression and Broad Dynamic Range

Tools for regulated gene expression in Enterococcus faecalis are extremely limited. In this report, we describe the construction of an expression vector for E. faecalis, designated pCIE, utilizing the P(Q) pheromone-responsive promoter of plasmid pCF10. We demonstrate that this promoter is tightly r...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Main Authors: Weaver, Keith E., Chen, Yuqing, Miiller, Elly M., Johnson, Jake N., Dangler, Alex A., Manias, Dawn A., Clem, Aaron M., Schjodt, Daniel J., Dunny, Gary M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2017
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5446624/
https://ncbi.nlm.nih.gov/pubmed/28348028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00065-17
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