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In vitro transcription of two rRNA genes of the archaebacterium Sulfolobus sp. B12 indicates a factor requirement for specific initiation.

We describe a cell-free transcription system for the archaebacterium Sulfolobus sp. B12 that specifically initiates transcription at the 5S rRNA-encoding DNA and the 16S/23S rRNA-encoding DNA promoters of the same species. With this crude extract system, specific initiation was absolutely dependent...

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Bibliographic Details
Main Authors: Hüdepohl, U, Reiter, W D, Zillig, W
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC54426/
https://ncbi.nlm.nih.gov/pubmed/2116009
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