Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis

Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. Depending on the application, background DNA from WGA kits can be problematic. Three WGA kits were tested for their uti...

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Bibliographische Detailangaben
Veröffentlicht in:J Clin Microbiol
Hauptverfasser: Thoendel, Matthew, Jeraldo, Patricio, Greenwood-Quaintance, Kerryl E., Yao, Janet, Chia, Nicholas, Hanssen, Arlen D., Abdel, Matthew P., Patel, Robin
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2017
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5442535/
https://ncbi.nlm.nih.gov/pubmed/28356418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02402-16
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