Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis

Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. Depending on the application, background DNA from WGA kits can be problematic. Three WGA kits were tested for their uti...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Main Authors: Thoendel, Matthew, Jeraldo, Patricio, Greenwood-Quaintance, Kerryl E., Yao, Janet, Chia, Nicholas, Hanssen, Arlen D., Abdel, Matthew P., Patel, Robin
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2017
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5442535/
https://ncbi.nlm.nih.gov/pubmed/28356418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02402-16
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