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Use of glnQ as a Counterselectable Marker for Creation of Allelic Exchange Mutations in Group B Streptococci
Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ. We have used this phenomenon to create a counterselectable marker system f...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC544214/ https://ncbi.nlm.nih.gov/pubmed/15640242 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.1.587-590.2005 |
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