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Effect of oligonucleotide primers in determining viral variability within hosts

BACKGROUND: Genetic variability in viral populations is usually estimated by means of polymerase chain reaction (PCR) based methods in which the relative abundance of each amplicon is assumed to be proportional to the frequency of the corresponding template in the initial sample. Although bias in te...

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Autors principals: Bracho, Maria Alma, García-Robles, Inmaculada, Jiménez, Nuria, Torres-Puente, Manuela, Moya, Andrés, González-Candelas, Fernando
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC543450/
https://ncbi.nlm.nih.gov/pubmed/15588294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-1-13
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