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Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system

The γ-aminobutyric acid (GABA) transporter 1 (GAT1) belongs to a family of Na(+) and Cl(−)-coupled transport proteins and possesses 12 putative transmembrane domains. To perform structural analyses of the GAT1 protein, the GAT1/green fluorescent protein (GFP) fusion protein was functionally expresse...

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Detalhes bibliográficos
Publicado no:Beilstein J Org Chem
Main Authors: Hu, Jing, Weise, Chris, Böttcher, Christoph, Fan, Hua, Yin, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: Beilstein-Institut 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5433199/
https://ncbi.nlm.nih.gov/pubmed/28546845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3762/bjoc.13.88
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