In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain

In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme as...

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Detalhes bibliográficos
Publicado no:Front Cell Neurosci
Main Authors: Cubas-Nuñez, Laura, Duran-Moreno, María, Castillo-Villalba, Jessica, Fuentes-Maestre, Jorge, Casanova, Bonaventura, García-Verdugo, José M., Gil-Perotín, Sara
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5433152/
https://ncbi.nlm.nih.gov/pubmed/28559798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fncel.2017.00141
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