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Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR

BACKGROUND: Diagnosis of enterotoxigenic E. coli (ETEC) associated diarrhea is complicated by the diversity of E.coli virulence factors. This study developed a multiplex quantitative PCR assay based on high-resolution melting curves analysis (HRM-qPCR) to identify and quantify genes encoding five ET...

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Udgivet i:BMC Microbiol
Main Authors: Wang, Weilan, Zijlstra, Ruurd T., Gänzle, Michael G.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5433089/
https://ncbi.nlm.nih.gov/pubmed/28506262
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-017-1023-5
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