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Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni(2+)

Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c)...

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Détails bibliographiques
Auteur principal: Polacco, Joseph Carmine
Format: Artigo
Langue:Inglês
Publié: 1977
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC543304/
https://ncbi.nlm.nih.gov/pubmed/16659950
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