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Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch

[FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG. According to the current models, HydF has the key double role of scaffold, upon wh...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Galazzo, Laura, Maso, Lorenzo, De Rosa, Edith, Bortolus, Marco, Doni, Davide, Acquasaliente, Laura, De Filippis, Vincenzo, Costantini, Paola, Carbonera, Donatella
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5431965/
https://ncbi.nlm.nih.gov/pubmed/28490758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-01886-y
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