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Agarose Spot as a Comparative Method for in situ Analysis of Simultaneous Chemotactic Responses to Multiple Chemokines

We describe a novel protocol to quantitatively and simultaneously compare the chemotactic responses of cells towards different chemokines. In this protocol, droplets of agarose gel containing different chemokines are applied onto the surface of a Petri dish, and then immersed under culture medium in...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ahmed, Mohaned, Basheer, Haneen A., Ayuso, Jose M., Ahmet, Djevdet, Mazzini, Marco, Patel, Roshan, Shnyder, Steven D., Vinader, Victoria, Afarinkia, Kamyar
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5430824/
https://ncbi.nlm.nih.gov/pubmed/28432337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-00949-4
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