Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR

Establishing a cure for HIV is hindered by the persistence of latently infected cells which constitute the viral reservoir. Real-time qPCR, used for quantification of this reservoir by measuring HIV DNA, requires external calibration; a common choice of calibrator is the 8E5 cell line, which is assu...

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Vydáno v:Sci Rep
Hlavní autoři: Busby, Eloise, Whale, Alexandra S., Ferns, R. Bridget, Grant, Paul R., Morley, Gary, Campbell, Jonathan, Foy, Carole A., Nastouli, Eleni, Huggett, Jim F., Garson, Jeremy A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2017
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5430807/
https://ncbi.nlm.nih.gov/pubmed/28446770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-01221-5
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