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Unusual semi‐extractability as a hallmark of nuclear body‐associated architectural noncoding RNAs
NEAT1_2 long noncoding RNA (lncRNA) is the molecular scaffold of paraspeckle nuclear bodies. Here, we report an improved RNA extraction method: extensive needle shearing or heating of cell lysate in RNA extraction reagent improved NEAT1_2 extraction by 20‐fold (a property we term “semi‐extractabilit...
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| Pubblicato in: | EMBO J |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
John Wiley and Sons Inc.
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5430218/ https://ncbi.nlm.nih.gov/pubmed/28404604 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201695848 |
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