Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum

A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower...

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Bibliographic Details
Main Authors: Edwards, Gerald E., Lilley, Ross McC., Craig, Stewart, Hatch, Marshall D.
Format: Artigo
Language:Inglês
Published: 1979
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC542927/
https://ncbi.nlm.nih.gov/pubmed/16660820
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