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Isolation and Cell-free Translation of Total Messenger RNA from Germinating Castor Bean Endosperm

Polyadenylated RNA was isolated from the total RNA fraction extracted from the endosperm tissue of 3-day-old castor bean seedlings by affinity chromatography on oligo(dT)-cellulose. This polyadenylated RNA was efficiently translated into protein when added to a messenger RNA-dependent cell-free syst...

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Détails bibliographiques
Publié dans:Plant Physiol
Auteurs principaux: Bowden-Bonnett, Linda, Lord, J. Michael
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1979
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC542914/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16660809/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.63.4.769
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