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Binding of ColE1-kan Plasmid DNA by Tobacco Protoplasts: Nonexpression of Plasmid Gene
Protoplasts prepared from cultured tobacco cells were treated with ColE1-kan plasmid DNA, a hybrid of ColE1 and pSC105 plasmids bearing a gene for kanamycin resistance. The conditions employed permitted the uptake or irreversible binding of 2.9% of the added DNA in acid-insoluble form. Upon commence...
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1979
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC542896/ https://ncbi.nlm.nih.gov/pubmed/16660791 |
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