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Molecular Counting with Localization Microscopy: A Bayesian Estimate Based on Fluorophore Statistics

Superresolved localization microscopy has the potential to serve as an accurate, single-cell technique for counting the abundance of intracellular molecules. However, the stochastic blinking of single fluorophores can introduce large uncertainties into the final count. Here we provide a theoretical...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Nino, Daniel, Rafiei, Nafiseh, Wang, Yong, Zilman, Anton, Milstein, Joshua N.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5425379/
https://ncbi.nlm.nih.gov/pubmed/28494949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.03.020
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