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lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

The membrane topology of Escherichia coli lac permease was analyzed using a set of 36 lac permease-alkaline phosphatase (lacY-phoA) gene fusions. The level of enzymatic activity of alkaline phosphatase fused to a cytoplasmic membrane protein appears to reflect whether the fusion junction site normal...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Principais autores: Calamia, J, Manoil, C
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1990
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54236/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2164211/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.13.4937
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