Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves
A procedure was developed for extracting polysomes from tobacco (Nicotiana sp) leaves. Unexpanded leaves ground in a medium consisting of 200 mm tris-HCl, pH 9, 400 mm KCl, 200 mm sucrose, and 35 mm MgCl(2) yielded larger amounts of polysomes with less degradation than polysomes from leaves extracte...
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| Vydáno v: | Plant Physiol |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1976
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC541953/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16659423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.57.1.5 |
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