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Isolation of Nucleoli and Localization of Ribonucleic Acid Polymerase I from Soybean Hypocotyl

An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the α-amanitin-insensitive RNA synthetic activity. This activity chromatographed...

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Detalhes bibliográficos
Main Authors: Lin, Chu-Yung, Guilfoyle, Thomas J., Chen, Yih-Ming, Key, Joe L.
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC541939/
https://ncbi.nlm.nih.gov/pubmed/16659409
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