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Imaging of DNA sequences with chemiluminescence.

We have coupled a chemiluminescent detection method that uses an alkaline phosphatase label to the genomic DNA sequencing protocol of Church and Gilbert [Church, G. M. & Gilbert, W. (1984) Proc. Natl. Acad. Sci. USA 81, 1991-1995]. Images of sequence ladders are obtained on x-ray film with exposure...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Tizard, R, Cate, R L, Ramachandran, K L, Wysk, M, Voyta, J C, Murphy, O J, Bronstein, I
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54146/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2191292/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.12.4514
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