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Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR
Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin–streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplificat...
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| Pubblicato in: | Biotechnol Appl Biochem |
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| Autori principali: | , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
John Wiley and Sons Inc.
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5412896/ https://ncbi.nlm.nih.gov/pubmed/26671010 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bab.1467 |
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