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A Microplate Reader-Based System for Visualizing Transcriptional Activity During in vivo Microbial Interactions in Space and Time

Here, we report the development of a microplate reader-based system for visualizing gene expression dynamics in living bacterial cells in response to a fungus in space and real-time. A bacterium expressing the red fluorescent protein mCherry fused to the promoter region of a regulator gene nunF indi...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Hennessy, Rosanna C., Stougaard, Peter, Olsson, Stefan
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5412646/
https://ncbi.nlm.nih.gov/pubmed/28325928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-00296-4
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