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Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling.

Conditions were devised to demonstrate GTP-regulated coupling between the yeast STE2-encoded receptor and its cognate guanine nucleotide-binding protein (G protein). Treatment of partially purified membranes with guanosine 5'-[gamma-thio]triphosphate (GTP[gamma-S]) converted the receptor from a...

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Bibliografiske detaljer
Main Authors: Blumer, K J, Thorner, J
Format: Artigo
Sprog:Inglês
Udgivet: 1990
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC54110/
https://ncbi.nlm.nih.gov/pubmed/2161538
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