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Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes

Here we describe a protocol that enables to automatically perform time-lapse imaging of growing root tips for several hours. Plants roots expressing fluorescent proteins or stained with dyes are imaged while they grow using automatic movement of the microscope stage that compensates for root growth...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Doumane, Mehdi, Lionnet, Claire, Bayle, Vincent, Jaillais, Yvon, Caillaud, Marie-Cécile
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409507/
https://ncbi.nlm.nih.gov/pubmed/28459086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2245
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