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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Monotopic proteins exert their function when attached to a membrane surface, and such interactions depend on the specific lipid composition and on the availability of enough area to perform the function. Nanodiscs are used to provide a membrane surface of controlled size and lipid content. In the ab...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: B. Kumar, Ramakrishnan, Zhu, Lin, Hebert, Hans, Jegerschöld, Caroline
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409284/
https://ncbi.nlm.nih.gov/pubmed/28287545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55148
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