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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Studies of integral membrane proteins in vitro are frequently complicated by the presence of a hydrophobic transmembrane domain. Further complicating these studies, reincorporation of detergent-solubilized membrane proteins into liposomes is a stochastic process where protein topology is impossible...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5409191/ https://ncbi.nlm.nih.gov/pubmed/28117823 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54971 |
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