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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

When novel proteins are identified through affinity-based isolation and bioinformatics analysis, they are often largely uncharacterized. Antibodies against specific peptides within the predicted sequence allow some localization experiments. However, other possible interactions with the antibodies of...

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Publicat a:J Vis Exp
Autors principals: Farwell, Sara Lynn N., Slee, Joshua B., Li, Yaqiu, Lowe-Krentz, Linda J.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408704/
https://ncbi.nlm.nih.gov/pubmed/28287514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55053
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