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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exh...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Bertin, Enrica, Piccoli, Martina, Franzin, Chiara, Nagy, Andras, Mileikovsky, Maria, De Coppi, Paolo, Pozzobon, Michela
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5407613/
https://ncbi.nlm.nih.gov/pubmed/28287531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54598
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