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Enhanced resolution of DNA restriction fragments: a procedure by two-dimensional electrophoresis and double-labeling.

A probe-free method was developed to detect DNA rearrangement in bacteria based on the electrophoretic separation of twice-digested restriction fragments of genomic DNA into a two-dimensional (2-D) pattern. The first restriction enzyme digestion was done in solution, followed by electrophoresis of t...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Yi, M, Au, L C, Ichikawa, N, Ts'o, P O
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54015/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2140194/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.10.3919
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