PCR Amplification with Primers Based on IS2404 and GC-Rich Repeated Sequence Reveals Polymorphism in Mycobacterium ulcerans

We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Ablordey, Anthony, Kotlowski, Roman, Swings, Jean, Portaels, Françoise
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2005
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC540136/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15635012/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.43.1.448-451.2005
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