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Blending DNA binding dyes to improve detection in real-time PCR
The success of real-time PCR (qPCR) analysis is partly limited by the presence of inhibitory compounds in the nucleic acid samples. For example, humic acid (HA) from soil and aqueous sediment interferes with amplification and also quenches the fluorescence of double-stranded (ds) DNA binding dyes, t...
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| 出版年: | Biotechnol Rep (Amst) |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Elsevier
2017
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5397098/ https://ncbi.nlm.nih.gov/pubmed/28459006 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2017.02.002 |
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