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Biochemical and molecular characterization of the isocitrate dehydrogenase with dual coenzyme specificity from the obligate methylotroph Methylobacillus Flagellatus

The isocitrate dehydrogenase (MfIDH) with unique double coenzyme specificity from Methylobacillus flagellatus was purified and characterized, and its gene was cloned and overexpressed in E. coli as a fused protein. This enzyme is homodimeric,—with a subunit molecular mass of 45 kDa and a specific ac...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Romkina, Anastasia Y., Kiriukhin, Michael Y.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5397045/
https://ncbi.nlm.nih.gov/pubmed/28423051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0176056
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